Seminarium Optyczne

Nonlinear microscopy has revolutionized biological imaging, enabling high-resolution visualization of complex samples. Unlike linear techniques, e.g., multiphoton microscopy offers intrinsic optical sectioning, reduced photodamage, and deeper tissue penetration. However, biological tissues are highly scattering, which fundamentally limits imaging depth and resolution. Overcoming this challenge is crucial for applications ranging from neuroscience to oncology, where clear visualization of deep structures is essential.I will present the three setups for nonlinear imaging: Two-photon microscope that we use for in-vivo imaging, temporal-focusing with super-resolution optical fluctuation imaging for quick and precise imaging, and speckle scanning microscope that together with nonlinearity shows a promise in overcoming the strong scattering.