Seminarium Zakładu Biofizyki

Syntetyczne 5’-końcowe fragmenty mRNA stanowią cenne narzędzia badawcze w biochemii i biologii molekularnej. W prezentacji zostaną omówione w zarysie metody wprowadzania struktury kapu do krótkich oligorybonukleotydów na podstawie badań własnych i doniesień literaturowych.

The 5’ end of eukaryotic mRNA (cap) carries a N7-methylguanosine residue linked by a 5’-5’ triphosphate bond. In vitro synthesis of cappped RNA is an important bottleneck for many biological studies. The lack of methods allowing the synthesis of large amounts of mRNA with a specific 5’-end sequence has hampered biological and structural studies. Due to the chemical nature of N7-methylguanosine, the synthesis of RNAs possessing a cap structure at the 5’ end is still a significant challenge. Some aspects of the problem will be presented during seminar.