Seminarium Zakładu Biofizyki

Decapping scavenger enzyme (DcpS) is required for the removal of the 5'-end m7GpppN cap of mRNAs what is a crucial step in the regulation of mRNA stability and gene expression. These enzymes are homodimers. We investigate binding of substrate and product analogues of the mRNA cap, m7Gp(CH2)ppG and m7GMP, respectively, by human DcpS wild type (DcpS-WT/WT) and its mutant, with one binding site compromised (DcpS-WT/BC), using stopped-flow fluorimetry. Binding process by each active site and for each ligand is composed of formation of an encounter complex followed by conformational transitions. We observe substantial differences between both ligands with respect to the rate constants and details of the mechanism of binding which might be of biological relevance. They will be discussed during the lecture (seminarium będzie wygłoszone po angielsku)

Decapping scavenger enzyme (DcpS) is required for the removal of the 5'-end m7GpppN cap of mRNAs what is a crucial step in the regulation of mRNA stability and gene expression. These enzymes are homodimers. We investigate binding of substrate and product analogues of the mRNA cap, m7Gp(CH2)ppG and m7GMP, respectively, by human DcpS wild type (DcpS-WT/WT) and its mutant, with one binding site compromised (DcpS-WT/BC), using stopped-flow fluorimetry. Binding process by each active site and for each ligand is composed of formation of an encounter complex followed by conformational transitions. We observe substantial differences between both ligands with respect to the rate constants and details of the mechanism of binding which might be of biological relevance. They will be discussed during the lecture.