Seminarium Optyczne

Single fluorescent emitters in biological samples are probably the most common sources of quantumlight. Nevertheless, their quantum optical properties are rarely exploited. I will discuss how fluorescencemicroscopy can benefit from measurements of quantum correlations. Such measurements allowed countingemitters within a diffraction-limited spot [1] and enhancing the resolution of classical super-resolutionmethods further beyond the diffraction limit, as in the case of recently introduced Quantum Image ScanningMicroscopy (QISM) [2].We found that the classical analog of QISM relying on classical light correlations offers a higher SNR at shortmeasurement times and is less demanding experimentally. This method, termed Super-resolution opticalfluctuation image scanning microscopy (SOFISM) [3], exploits fluorescent emitter blinking as its imagecontrast. SOFISM offers a robust path to achieve high-resolution images with a slightly modified confocalmicroscope, using standard fluorescent labels and reasonable acquisition times.[1] Y. Israel, et al., Quantum correlation enhanced super-resolution localization microscopy enabled by a fibrebundle camera. Nat.Comm. 8, 14786 (2017).[2] R. Tenne, et al., Super-resolution enhancement by quantum image scanning microscopy, Nat. Phot., 13,116–122 (2019).[3] A. Sroda, et al., SOFISM: Super-resolution optical fluctuation image scanning microscopy, SOFISM:Super-resolution optical fluctuation image scanning microscopy, Optica 7, 1308-1316 (2020).

Seminarium z użyciem połączenia internetowegohttps://zoom.us/j/97696726563(meeting ID: ID 97696726563, password: 314297)