Seminarium Optyczne

Lensless digital holographic microscopy (LDHM), as one of key computational microscopy techniques, performs high-throughput in silico imaging. Numerical propagation of digitally recorded in-line Gabor holograms allows for accessing both amplitude (absorption) and phase (refraction) contrast, devoid of microscope objective limitations, e.g., in depth of field and field of view. The in-line coherent holographic framework induces inherent twin image errors and various coherent artifacts, however. The signal-to-noise ratio of reconstructed holograms additionally deteriorates due to low photon budget environment, favorable in terms of time-lapse photostimulation-free bioimaging of live cells. I plan to discuss several techniques for minimization of LDHM reconstruction errors, and present bio-applications of enhanced LDHM in dynamic (e.g., migrating neural cells) and static (brain tissue slices) scenarios."